• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30339-1-AP

    WB analysis using 30339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30339-1-AP (Clusterin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 30339-1-AP

    IHC staining of human liver using 30339-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30339-1-AP (Clusterin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 30339-1-AP

    IHC staining of human prostate cancer using 30339-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30339-1-AP (Clusterin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 30339-1-AP

    IF Staining of HeLa using 30339-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (30339-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30339-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ApoJalpha, Apo-J, APOJ, Apo J, Aging-associated gene 4 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman plasma, mouse serum
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30339-1-AP targets Clusterin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clusterin
别名 ApoJalpha, Apo-J, APOJ, Apo J, Aging-associated gene 4 protein
计算分子量 449 aa, 53 kDa
观测分子量34-39 kDa
GenBank蛋白编号BC010514
基因名称 CLU
Gene ID (NCBI) 1191
RRIDAB_3669713
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10909
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

实验方案

Product Specific Protocols
WB protocol for Clusterin antibody 30339-1-APDownload protocol
IHC protocol for Clusterin antibody 30339-1-APDownload protocol
IF protocol for Clusterin antibody 30339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols