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Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30339-1-AP

Print datasheet

Synonyms

ApoJalpha, Apo-J, APOJ, Apo J, Aging-associated gene 4 protein

验证数据展示

  • WB analysis using 30339-1-AP

    WB analysis using 30339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30339-1-AP (Clusterin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 30339-1-AP

    IHC staining of human liver using 30339-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30339-1-AP (Clusterin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 30339-1-AP

    IHC staining of human prostate cancer using 30339-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30339-1-AP (Clusterin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 30339-1-AP

    IF Staining of HeLa using 30339-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (30339-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inhuman plasma, mouse serum
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30339-1-AP targets Clusterin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clusterin
Synonyms ApoJalpha, Apo-J, APOJ, Apo J, Aging-associated gene 4 protein
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight34-39 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

实验方案

Product Specific Protocols
WB protocol for Clusterin antibody 30339-1-APDownload protocol
IHC protocol for Clusterin antibody 30339-1-APDownload protocol
IF protocol for Clusterin antibody 30339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols