• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Collagen Type II Polyclonal antibody

Collagen Type II Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

IHC, IF/ICC, IF-P, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 28459-1-AP

Print datasheet

Synonyms

COL2, COL2A1, COL11A3, Chondrocalcin, AOM

验证数据展示

  • IHC staining of human lung using 28459-1-AP

    IHC staining of human lung using 28459-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL2A1 antibody included
  • IHC staining of human lung using 28459-1-AP

    IHC staining of human lung using 28459-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL2A1 antibody included
  • IF Staining of human lung cancer using 28459-1-AP

    IF Staining of human lung cancer using 28459-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human lung cancer using 28459-1-AP

    IF Staining of human lung cancer using 28459-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HEK-293 using 28459-1-AP

    IF Staining of HEK-293 using 28459-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Collagen Type II antibody (28459-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy COL2A1 Ready-To-Use IHC Kit. COL2A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28459-1-AP targets Collagen Type II in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, rabbit
Immunogen Collagen Type II fusion protein Ag29265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type II, alpha 1
Synonyms COL2, COL2A1, COL11A3, Chondrocalcin, AOM
Calculated Molecular Weight 134 kDa
GenBank Accession NumberBC007252
Gene Symbol COL2A1
Gene ID (NCBI) 1280
RRIDAB_2881147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02458
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Collagen Type II antibody 28459-1-APDownload protocol
IF protocol for Collagen Type II antibody 28459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
View Article
ratIF

Adv Mater

Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy

Authors - Manyu Chen
View Article
ratIF

Biomaterials

Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration

Authors - Lei Liu
View Article
humanWB

Nat Commun

Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.

Authors - Yun Zhang
View Article
humanWB

J Musculoskelet Neuronal Interact

Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

Authors - Xiaoming Shi
View Article
rabbitIHC

Acta Biomater

The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage.

Authors - Wang Changchen
View Article