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Collagen Type V Polyclonal antibody

Collagen Type V Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28382-1-AP

Print datasheet

Synonyms

COL5A1, Collagen alpha 1(V) chain, Collagen Type V, collagen, type V, alpha 1

验证数据展示

  • IHC staining of human colon using 28382-1-AP

    IHC staining of human colon using 28382-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL5A1 antibody included
  • IHC staining of human colon using 28382-1-AP

    IHC staining of human colon using 28382-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL5A1 antibody included

经过测试的应用

Positive IHC detected inhuman colon tissue, human lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COL5A1 Ready-To-Use IHC Kit. COL5A1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28382-1-AP targets Collagen Type V in IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Human
Immunogen Purified native protein corresponding to human collagen type V 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type V, alpha 1
Synonyms COL5A1, Collagen alpha 1(V) chain, Collagen Type V, collagen, type V, alpha 1
Calculated Molecular Weight 184 kDa
GenBank Accession NumberNM_000093
Gene Symbol COL5A1
Gene ID (NCBI) 1289
RRIDAB_2881129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Collagen Type V antibody 28382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols