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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 31280-1-AP

    WB analysis using 31280-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31280-1-AP (COL6A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Collagen Type VI Polyclonal antibody

Collagen Type VI Polyclonal Antibody for WB, ELISA
Cat No. 31280-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COL6A3, Collagen alpha-3(VI) chain, Collagen alpha 3(VI) chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31280-1-AP targets Collagen Type VI in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Collagen Type VI fusion protein Ag35065 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type VI, alpha 3
Synonyms COL6A3, Collagen alpha-3(VI) chain, Collagen alpha 3(VI) chain
Calculated Molecular Weight344 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_004369
Gene Symbol COL6A3
Gene ID (NCBI) 1293
RRIDAB_3669927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.

实验方案

Product Specific Protocols
WB protocol for Collagen Type VI antibody 31280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols