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验证数据展示

  • WB analysis using 24401-1-AP

    WB analysis using 24401-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24401-1-AP (Collagen Type XXVIII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 24401-1-AP

    IHC staining of human stomach cancer using 24401-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24401-1-AP (Collagen Type XXVIII antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 24401-1-AP

    IF Staining of U2OS using 24401-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Collagen Type XXVIII antibody (24401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Collagen Type XXVIII Polyclonal antibody

Collagen Type XXVIII Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24401-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

COL28A1, Collagen alpha-1(XXVIII) chain, Collagen alpha 1(XXVIII) chain, COL28

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24401-1-AP targets Collagen Type XXVIII in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Collagen Type XXVIII fusion protein Ag21459 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type XXVIII, alpha 1
Synonyms COL28A1, Collagen alpha-1(XXVIII) chain, Collagen alpha 1(XXVIII) chain, COL28
Calculated Molecular Weight 1125 aa, 117 kDa
Observed Molecular Weight72 kDa, 100-117 kDa
GenBank Accession NumberBC136892
Gene Symbol COL28A1
Gene ID (NCBI) 340267
RRIDAB_3669435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2UY09
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL28A1 (collagen type XXVIII alpha 1 chain), also known as COL28. It is expected to be located in the basement membranes, cytoplasm and nucleus. And the protein is mainly expressed in salivary gland and adrenal. This antibody can recognize the 117 kDa and 72 kDa isoforms of target. COL28A1 is a new collagen that cannot clearly be assigned to any collagen subgroup, despite the fact that it contains VWA domains. Ongoing investigations will shed further light on the role of COL28A1 in the assembly of nerve fiber basement membranes. Although no human mutation has been associated with COL28A1, it is tempting to speculate that mutations in this gene would lead to neurodegenerative disease (PMID: 16330543).

实验方案

Product Specific Protocols
WB protocol for Collagen Type XXVIII antibody 24401-1-APDownload protocol
IF protocol for Collagen Type XXVIII antibody 24401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols