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Complement factor B Monoclonal antibody, PBS Only

Complement factor B Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

8D2B6

Cat No : 66154-1-PBS

Print datasheet

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

验证数据展示

  • WB analysis of human plasma using 66154-1-Ig (same clone as 66154-1-PBS)

    WB analysis of human plasma using 66154-1-Ig (same clone as 66154-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66154-1-Ig (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

66154-1-PBS targets Complement factor B in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human
Immunogen Complement factor B fusion protein Ag19107 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name complement factor B
Synonyms BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC007990
Gene Symbol CFB
Gene ID (NCBI) 629
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP00751
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.