验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
51037-2-AP targets Connexin-26 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | gap junction protein, beta 2, 26kDa |
别名 | Connexin 26, Connexin-26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, Gap junction beta 2 protein, GJB2, HID, KID, NSRD1, PPK |
计算分子量 | 26 kDa |
观测分子量 | 26 kDa |
GenBank蛋白编号 | BC017048 |
基因名称 | Connexin-26 |
Gene ID (NCBI) | 2706 |
RRID | AB_2110785 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P29033 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin-26 (GJB2) is a beta class gap junction protein. Mutations in the gene for connexin-26 are responsible for as much as 50% of pre-lingual, recessive deafness.
实验方案
Product Specific Protocols | |
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WB protocol for Connexin-26 antibody 51037-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |