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Connexin 43 Recombinant antibody, PBS Only

Connexin 43 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3O6

Cat no : 80543-1-PBS

Print datasheet

Synonyms

GJA1, Connexin-43, 3O6, Gap junction alpha-1 protein, Gap junction alpha 1 protein

验证数据展示

  • WB analysis using 80543-1-RR (same clone as 80543-1-PBS)

    WB analysis using 80543-1-RR (same clone as 80543-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80543-1-RR (Connexin 43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GJA1/Connexin 43 antibody included
  • IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GJA1/Connexin 43 antibody included
  • IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80543-1-RR

    Affinity and Kinetic Characterization of 80543-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80543-1-RR against Human Connexin 43 were performed. The affinity constant is 5.19 nM.

  • IF Staining of HeLa using 80543-1-RR (same clone as 80543-1-PBS)

    IF Staining of HeLa using 80543-1-RR (same clone as 80543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GJA1/Connexin 43 Ready-To-Use IHC Kit. GJA1/Connexin 43 primary antibody included.

产品信息

80543-1-PBS targets Connexin 43 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Connexin 43 fusion protein Ag25533 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gap junction protein, alpha 1, 43kDa
Synonyms GJA1, Connexin-43, 3O6, Gap junction alpha-1 protein, Gap junction alpha 1 protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC026329
Gene Symbol Connexin-43
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17302
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).