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Cortactin Recombinant antibody

Cortactin Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84569-1-RR

产品说明书

CloneNo. 241987A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

CTTN, SRC8, EMS1, 241987A12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HAP1 cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy Cortactin Ready-To-Use IHC Kit. Cortactin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84569-1-RR targets Cortactin in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Cortactin fusion protein Ag1940 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cortactin
别名 CTTN, SRC8, EMS1, 241987A12
计算分子量 57 kDa
观测分子量70-85 kDa
GenBank蛋白编号BC008799
基因名称 Cortactin
Gene ID (NCBI) 2017
RRIDAB_3672073
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14247
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

实验方案

Product Specific Protocols
WB protocol for Cortactin antibody 84569-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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