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验证数据展示

  • WB analysis using 84569-1-RR

    WB analysis using 84569-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84569-1-RR (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HAP1 using 84569-1-RR

    WB analysis of HAP1 using 84569-1-RR

    WB result of Cortactin antibody (84569-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and Cortactin knockout HAP1 cells.

  • Affinity and Kinetic Characterization of 84569-1-RR

    Affinity and Kinetic Characterization of 84569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84569-1-RR against Human Cortactin were performed. The affinity constant is 7.44 nM.

Cortactin Recombinant antibody

Cortactin Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84569-1-RR

Print datasheet

CloneNo. 241987A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CTTN, SRC8, EMS1, 241987A12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy Cortactin Ready-To-Use IHC Kit. Cortactin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84569-1-RR targets Cortactin in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Cortactin fusion protein Ag1940 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cortactin
Synonyms CTTN, SRC8, EMS1, 241987A12
Calculated Molecular Weight 57 kDa
Observed Molecular Weight70-85 kDa
GenBank Accession NumberBC008799
Gene Symbol Cortactin
Gene ID (NCBI) 2017
RRIDAB_3672073
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14247
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

实验方案

Product Specific Protocols
WB protocol for Cortactin antibody 84569-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols