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CstF-64 Monoclonal antibody

CstF-64 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C5G2

Cat no : 68520-1-Ig

Print datasheet

Synonyms

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2

验证数据展示

  • WB analysis using 68520-1-Ig

    WB analysis using 68520-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68520-1-Ig (CstF-64 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CSTF2 Ready-To-Use IHC Kit. CSTF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68520-1-Ig targets CstF-64 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CstF-64 fusion protein Ag33751 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Synonyms CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2
Calculated Molecular Weight 61 kDa
Observed Molecular Weight64-70 kDa
GenBank Accession NumberBC017712
Gene Symbol CstF-64
Gene ID (NCBI) 1478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP33240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

实验方案

Product Specific Protocols
WB protocol for CstF-64 antibody 68520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols