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Species Reactivity
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验证数据展示

  • WB analysis using 30322-1-AP

    WB analysis using 30322-1-AP

    Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 30322-1-AP (Cxcl1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CXCL1 Polyclonal antibody

CXCL1 Polyclonal Antibody for WB, ELISA
Cat No. 30322-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Growth-regulated alpha protein, chemokine (C X C motif) ligand 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30322-1-AP targets CXCL1 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-X-C motif) ligand 1
Synonyms Growth-regulated alpha protein, chemokine (C X C motif) ligand 1
Observed Molecular Weight11 kDa
GenBank Accession NumberBC037997
Gene Symbol Cxcl1
Gene ID (NCBI) 14825
RRIDAB_3086293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12850
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils, and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils.

实验方案

Product Specific Protocols
WB protocol for CXCL1 antibody 30322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols