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  • Featured Product
  • KD/KO Validated

Cyclin A2 Monoclonal antibody, PBS Only

Cyclin A2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6C11

Cat no : 66391-1-PBS

Print datasheet

Synonyms

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

验证数据展示

  • WB analysis using 66391-1-Ig (same clone as 66391-1-PBS)

    WB analysis using 66391-1-Ig (same clone as 66391-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

    WB analysis of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

    WB result of Cyclin A2 antibody (66391-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

    IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNA2 antibody included
  • IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

    IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNA2 antibody included
  • IF Staining of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

    IF Staining of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CCNA2 Ready-To-Use IHC Kit. CCNA2 primary antibody included.

产品信息

66391-1-PBS targets Cyclin A2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Cyclin A2 fusion protein Ag12765 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cyclin A2
Synonyms CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2
Calculated Molecular Weight 432 aa, 49 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC104783
Gene Symbol Cyclin A2
Gene ID (NCBI) 890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20248
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.