• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Cyclin D1 Polyclonal antibody

Cyclin D1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26939-1-AP

Print datasheet

Synonyms

CCND1, cyclin D, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein

验证数据展示

  • WB analysis using 26939-1-AP

    WB analysis using 26939-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 26939-1-AP

    WB analysis using 26939-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 26939-1-AP

    IHC staining of human ovary tumor using 26939-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 26939-1-AP

    IHC staining of human ovary tumor using 26939-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of SH-SY5Y using 26939-1-AP

    FC experiment of SH-SY5Y using 26939-1-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Cyclin D1 (26939-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of MCF-7 using 26939-1-AP

    IF Staining of MCF-7 using 26939-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

  • IF Staining of NIH/3T3 using 26939-1-AP

    IF Staining of NIH/3T3 using 26939-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 26939-1-AP

    FC experiment of MCF-7 using 26939-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin D1 (26939-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis of HeLa using 26939-1-AP

    WB analysis of HeLa using 26939-1-AP

    Non-treated HeLa and thymidine treated HeLa cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, C6 cells, NIH/3T3 cells, HeLa cells, Neuro-2a cells, mouse lung tissue
Positive IHC detected inhuman ovary tumor tissue, human oesophagus cancer tissue, human lung cancer tissue, mouse spleen tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, NIH/3T3 cells
Positive FC (Intra) detected inSH-SY5Y cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26939-1-AP targets Cyclin D1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, bovine, sheep, goat
Immunogen Cyclin D1 fusion protein Ag25393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin D1
Synonyms CCND1, cyclin D, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein
Calculated Molecular Weight 295 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000076
Gene Symbol Cyclin D1
Gene ID (NCBI) 595
RRIDAB_2880691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24385
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

实验方案

Product Specific Protocols
WB protocol for Cyclin D1 antibody 26939-1-APDownload protocol
IHC protocol for Cyclin D1 antibody 26939-1-APDownload protocol
IF protocol for Cyclin D1 antibody 26939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cancer

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Authors - Xi Jin
View Article
humanWB,IHC

Adv Sci (Weinh)

Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation

Authors - Huan Li
View Article
humanWB

J Pineal Res

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Authors - Li Cui
View Article
humanIF

Theranostics

Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

Authors - Bojun Li
View Article
humanWB

Nat Commun

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

Authors - Dan Su
View Article
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
View Article