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验证数据展示

  • WB analysis of Raji using 66357-1-Ig (same clone as 66357-1-PBS)

    WB analysis of Raji using 66357-1-Ig (same clone as 66357-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66357-1-Ig (same clone as 66357-1-PBS)

    WB analysis of Jurkat using 66357-1-Ig (same clone as 66357-1-PBS)

    WB result of Cyclin D3 antibody (66357-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66357-1-Ig (same clone as 66357-1-PBS)

    IHC staining of human pancreas cancer using 66357-1-Ig (same clone as 66357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCND3 antibody included
  • IHC staining of human pancreas cancer using 66357-1-Ig (same clone as 66357-1-PBS)

    IHC staining of human pancreas cancer using 66357-1-Ig (same clone as 66357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCND3 antibody included
  • Featured Product
  • KD/KO验证

Cyclin D3 Monoclonal antibody, PBS Only

Cyclin D3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66357-1-PBS

产品说明书

CloneNo. 1F2C6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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66357-1-Ig

Planning an IHC experiment? We recommend our IHCeasy CCND3 Ready-To-Use IHC Kit. CCND3 primary antibody included.

产品信息

66357-1-PBS targets Cyclin D3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Cyclin D3 fusion protein Ag22825 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin D3
别名 CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3
计算分子量 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC011616
基因名称 Cyclin D3
Gene ID (NCBI) 896
ENSEMBL Gene IDENSG00000112576
RRIDAB_2881736
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30281
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activitiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.