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Cyclin E1 Recombinant antibody

Cyclin E1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240136D4

Cat no : 82700-10-RR

Print datasheet

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

验证数据展示

  • WB analysis of HT-29 using 82700-10-RR

    WB analysis of HT-29 using 82700-10-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82700-10-RR

    WB analysis using 82700-10-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 82700-10-RR

    WB analysis of rat heart using 82700-10-RR

    rat heart tissue were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 82700-10-RR

    FC experiment of MCF-7 using 82700-10-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using 82700-10-RR

    FC experiment of HeLa using 82700-10-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis using 82700-10-RR

    WB analysis using 82700-10-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82700-10-RR

    Affinity and Kinetic Characterization of 82700-10-RR

    Biolayer interferometry (BLl) kinetic assays of 82700-10-RR against Human Cyclin E were performed. The affinity constant is 0.564 nM.

经过测试的应用

Positive WB detected inHT-29 cells, MCF-7 cells, rat heart tissue, HuH-7 cells
Positive FC (Intra) detected inHeLa cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82700-10-RR targets Cyclin E1 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen Cyclin E1 fusion protein Ag2110 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cyclin E1
Synonyms CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE
Calculated Molecular Weight 410 aa, 47 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC035498
Gene Symbol Cyclin E1
Gene ID (NCBI) 898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24864
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

实验方案

Product Specific Protocols
WB protocol for Cyclin E1 antibody 82700-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols