• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HeLa using 82700-9-RR (same clone as 82700-9-PBS)

    FC experiment of HeLa using 82700-9-RR (same clone as 82700-9-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-9-RR, Clone:240136H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82700-9-RR (same clone as 82700-9-PBS)

    IF Staining of HepG2 using 82700-9-RR (same clone as 82700-9-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-9-RR, Clone: 240136H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 82700-9-RR (same clone as 82700-9-PBS)

    FC experiment of MCF-7 using 82700-9-RR (same clone as 82700-9-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-9-RR, Clone:240136H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82700-9-RR (same clone as 82700-9-PBS)

    IF Staining of HeLa using 82700-9-RR (same clone as 82700-9-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E antibody (82700-9-RR, Clone: 240136H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00361-1

    Cytometric bead array standard curve of MP00361-1

    Cytometric bead array standard curve of MP00361-1, Cyclin E1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82700-7-PBS. Detection antibody: 82700-9-PBS. Standard: Ag2110. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 82700-9-PBS

    Affinity and Kinetic Characterization of 82700-9-PBS

    Biolayer interferometry (BLl) kinetic assay of 82700-9-PBS against Human Cyclin E1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 82700-9-RR

    Affinity and Kinetic Characterization of 82700-9-RR

    Biolayer interferometry (BLl) kinetic assays of 82700-9 -RR against Human Cyclin E were performed. The affinity constant is below 1 pM.

Cyclin E1 Recombinant antibody, PBS Only (Detector)

Cyclin E1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 82700-9-PBS

产品说明书

CloneNo. 240136H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

82700-9-PBS targets Cyclin E1 as part of a matched antibody pair:

MP00361-1: 82700-7-PBS capture and 82700-9-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Cyclin E1 fusion protein Ag2110 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin E1
别名 CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE
计算分子量 410 aa, 47 kDa
GenBank蛋白编号BC035498
基因名称 Cyclin E1
Gene ID (NCBI) 898
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24864
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).