Cyclin H Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Cyclin H antibody (67065-1-Ig, Clone: 2C5G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin H (67065-1-Ig, Clone:2C5G11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.