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Cyp4a12a Polyclonal antibody

Cyp4a12a Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51157-1-AP

Print datasheet

Synonyms

Cyp4a12, Cyp4a12a

验证数据展示

  • WB analysis of mouse liver using 51157-1-AP

    WB analysis of mouse liver using 51157-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 51157-1-AP (Cyp4a12a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 51157-1-AP

    IHC staining of rat liver using 51157-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 51157-1-AP

    IHC staining of rat liver using 51157-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

51157-1-AP targets Cyp4a12a in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity mouse, rat, human
Cited Reactivityrat
Immunogen Cyp4a12a fusion protein Ag0509 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 4, subfamily a, polypeptide 12a
Synonyms Cyp4a12, Cyp4a12a
Observed Molecular Weight 58-70 kDa
GenBank Accession NumberNM_177406
Gene Symbol Cyp4a12a
Gene ID (NCBI) 277753
RRIDAB_2881255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ91WL5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. Mouse Cyp4a12a oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. For modification, the MW of Cyp4a12a is about 58-70 kDa in western blot detection.

实验方案

Product Specific Protocols
WB protocol for Cyp4a12a antibody 51157-1-APDownload protocol
IHC protocol for Cyp4a12a antibody 51157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Exp Cell Res

Effects of PPARs/20-HETE on the renal impairment under diabetic conditions.

Authors - Shumei Ding
View Article