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验证数据展示

  • WB analysis using 83422-1-RR

    WB analysis using 83422-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83422-1-RR (cystatin c antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 83422-1-RR

    IHC staining of mouse kidney using 83422-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 83422-1-RR

    IHC staining of mouse kidney using 83422-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83422-1-RR

    Affinity and Kinetic Characterization of 83422-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83422-1-RR against Mouse Cystatin C were performed. The affinity constant is 20.3 nM.

Cystatin C Recombinant antibody

Cystatin C Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83422-1-RR

产品说明书

CloneNo. 240202A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Cst3, CysC, 240202A2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83422-1-RR targets Cystatin C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cystatin C
别名 Cst3, CysC, 240202A2
计算分子量15KD
观测分子量 13-16 kDa
GenBank蛋白编号NM_009976.4
基因名称 Cystatin C
Gene ID (NCBI) 13010
RRIDAB_3671071
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP21460
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidney.

实验方案

Product Specific Protocols
WB protocol for Cystatin C antibody 83422-1-RRDownload protocol
IHC protocol for Cystatin C antibody 83422-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols