Cystatin S Polyclonal antibody
Cystatin S Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human saliva tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
The immunogen of 26979-1-AP is Cystatin S Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Cystatin S fusion protein Ag25619 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cystatin S |
| Synonyms | CST4, Cystatin 4, cystatin S, Cystatin SA III, Salivary acidic protein 1 |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC065714 |
| Gene Symbol | Cystatin S |
| Gene ID (NCBI) | 1472 |
| RRID | AB_2880710 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01036 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cystatins are a family of inhibitors of cysteine peptidases that comprises the salivary cystatins D and S-type (including CST5 and CST4,1,2) and cystatin C-type (CST3) (PMID: 25329717). The D and S-type cystatin have also been detected in seminal plasma, tears, and tracheobronchial fluid but not in other fluids and secretions where cystatin C is found. And the CST4 was expressed in the serous acinar and demilune cells of the human submandibular gland and at lower levels in the serous acini of the parotid gland (PMID:11879580). CST4 specifically combines with cysteine protease to regulate its activity, thus preventing hydrolysis of the extracellular matrix. And some studies propose that CST4 might be a biomarker for gastrointestinal cancer (PMID:29218251; PMID:29636621).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cystatin S antibody 26979-1-AP | Download protocol |
| IHC protocol for Cystatin S antibody 26979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Prognostic value of immune-related cells and genes in the tumor microenvironment of ovarian cancer, especially CST4. |