• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Cytochrome P450 Reductase Polyclonal antibody

Cytochrome P450 Reductase Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29814-1-AP

Print datasheet

Synonyms

POR, P450R, CYPOR, CPR

验证数据展示

  • WB analysis using 29814-1-AP

    WB analysis using 29814-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 29814-1-AP

    WB analysis of HepG2 using 29814-1-AP

    WB result of Cytochrome P450 Reductase antibody (29814-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome P450 Reductase transfected HepG2 cells.

经过测试的应用

Positive WB detected inhuman placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29814-1-AP targets Cytochrome P450 Reductase in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytochrome P450 Reductase fusion protein Ag30899 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name P450 (cytochrome) oxidoreductase
Synonyms POR, P450R, CYPOR, CPR
Calculated Molecular Weight 77 kDa
Observed Molecular Weight77 kDa
GenBank Accession NumberNM_000941
Gene Symbol Cytochrome P450 Reductase
Gene ID (NCBI) 5447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16435
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome P450 Reductase (CYPOR) consists of the flavin mononucleotide (FMN) domain and the flavin adenine dinucleotide (FAD) domain, which bind the corresponding cofactors and transfer electrons from NADPH via FAD and FMN (PMID: 33498551). CYPOR is an essential component of the microsomal P450 mixed-function oxidase system and is required for electron transfer from NADPH to cytochromes P450 in microsomes. The calculated molecular weight of CYPOR is 77 kDa.

实验方案

Product Specific Protocols
WB protocol for Cytochrome P450 Reductase antibody 29814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols