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Cytochrome P450 Reductase Polyclonal antibody

Cytochrome P450 Reductase Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29814-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

POR, P450R, CYPOR, CPR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 500 ug/ml

库存:现货


经过测试的应用

Positive WB detected inhuman placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29814-1-AP targets Cytochrome P450 Reductase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Cytochrome P450 Reductase fusion protein Ag30899 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 P450 (cytochrome) oxidoreductase
别名 POR, P450R, CYPOR, CPR
计算分子量 77 kDa
观测分子量77 kDa
GenBank蛋白编号NM_000941
基因名称 Cytochrome P450 Reductase
Gene ID (NCBI) 5447
RRIDAB_3086171
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16435
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome P450 Reductase (CYPOR) consists of the flavin mononucleotide (FMN) domain and the flavin adenine dinucleotide (FAD) domain, which bind the corresponding cofactors and transfer electrons from NADPH via FAD and FMN (PMID: 33498551). CYPOR is an essential component of the microsomal P450 mixed-function oxidase system and is required for electron transfer from NADPH to cytochromes P450 in microsomes. The calculated molecular weight of CYPOR is 77 kDa.

实验方案

Product Specific Protocols
WB protocol for Cytochrome P450 Reductase antibody 29814-1-APDownload protocol
IHC protocol for Cytochrome P450 Reductase antibody 29814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

AMPD3 promotes doxorubicin-induced cardiomyopathy through HSP90α-mediated ferroptosis

Authors - Liting Cheng
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