• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67185-1-Ig (same clone as 67185-1-PBS)

    WB analysis using 67185-1-Ig (same clone as 67185-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67185-1-Ig (CYTH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67185-1-Ig (same clone as 67185-1-PBS)

    IHC staining of human liver cancer using 67185-1-Ig (same clone as 67185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67185-1-Ig (same clone as 67185-1-PBS)

    IHC staining of human liver cancer using 67185-1-Ig (same clone as 67185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67185-1-Ig (same clone as 67185-1-PBS)

    IF Staining of HeLa using 67185-1-Ig (same clone as 67185-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Cytohesin 2 Monoclonal antibody, PBS Only

Cytohesin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67185-1-PBS

产品说明书

CloneNo. 3A7A1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

67185-1-PBS targets Cytohesin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Cytohesin 2 fusion protein Ag21279 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cytohesin 2
别名 ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC004361
基因名称 Cytohesin 2
Gene ID (NCBI) 9266
RRIDAB_2882481
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99418
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with small G proteins ARF1, ARF3, and ARF6.