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  • Featured Product
  • KD/KO Validated

Cytokeratin 13 Recombinant antibody

Cytokeratin 13 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230366B10

Cat no : 83058-1-RR

Print datasheet

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

验证数据展示

  • WB analysis using 83058-1-RR

    WB analysis using 83058-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83058-1-RR (KRT13 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human urothelial carcinoma using 83058-1-RR

    IHC staining of human urothelial carcinoma using 83058-1-RR

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • IHC staining of human urothelial carcinoma using 83058-1-RR

    IHC staining of human urothelial carcinoma using 83058-1-RR

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • WB analysis of A431 using 83058-1-RR

    WB analysis of A431 using 83058-1-RR

    WB result of Cytokeratin 13 antibody (83058-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells.

  • IF Staining of HaCaT using 83058-1-RR

    IF Staining of HaCaT using 83058-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83058-1-RR

    Affinity and Kinetic Characterization of 83058-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83058-1-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.99 nM.

经过测试的应用

Positive WB detected inHaCaT cells, A431 cells, mouse skin tissue, rat skin tissue, rat thymus tissue
Positive IHC detected inhuman urothelial carcinoma tissue, human cervical squamous cancer tissue, human oesophagus cancer tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83058-1-RR targets Cytokeratin 13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 13 fusion protein Ag0217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name keratin 13
Synonyms KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13
Calculated Molecular Weight 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC002661
Gene Symbol Cytokeratin 13
Gene ID (NCBI) 3860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13646
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 83058-1-RRDownload protocol
IHC protocol for Cytokeratin 13 antibody 83058-1-RRDownload protocol
IF protocol for Cytokeratin 13 antibody 83058-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols