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验证数据展示

  • WB analysis using 83058-1-RR

    WB analysis using 83058-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83058-1-RR (KRT13 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human urothelial carcinoma using 83058-1-RR

    IHC staining of human urothelial carcinoma using 83058-1-RR

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • IHC staining of human urothelial carcinoma using 83058-1-RR

    IHC staining of human urothelial carcinoma using 83058-1-RR

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • WB analysis of A431 using 83058-1-RR

    WB analysis of A431 using 83058-1-RR

    WB result of Cytokeratin 13 antibody (83058-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells.

  • IF Staining of HaCaT using 83058-1-RR

    IF Staining of HaCaT using 83058-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83058-1-RR

    Affinity and Kinetic Characterization of 83058-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83058-1-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.99 nM.

  • Featured Product
  • KD/KO验证

Cytokeratin 13 Recombinant antibody

Cytokeratin 13 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83058-1-RR

产品说明书

CloneNo. 230366B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHaCaT cells, A431 cells, mouse skin tissue, rat skin tissue, rat thymus tissue
Positive IHC detected inhuman urothelial carcinoma tissue, human cervical squamous cancer tissue, human oesophagus cancer tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83058-1-RR targets Cytokeratin 13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 13 fusion protein Ag0217 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 13
别名 KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13
计算分子量 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC002661
基因名称 Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_3670785
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13646
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 83058-1-RRDownload protocol
IHC protocol for Cytokeratin 13 antibody 83058-1-RRDownload protocol
IF protocol for Cytokeratin 13 antibody 83058-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols