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验证数据展示

  • IF Staining of A431 using 83058-4-RR

    IF Staining of A431 using 83058-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cytokeratin 13 antibody (83058-4-RR, Clone: 230366E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 83058-4-RR

    FC experiment of A431 using 83058-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT13 (83058-4-RR, Clone:230366E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83058-4-RR

    Affinity and Kinetic Characterization of 83058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83058-4-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.65 nM.

Cytokeratin 13 Recombinant antibody

Cytokeratin 13 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83058-4-RR

产品说明书

CloneNo. 230366E8

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IF/ICC, FC (Intra), ELISA

别名

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83058-4-RR targets Cytokeratin 13 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 Human
免疫原 Cytokeratin 13 fusion protein Ag0217 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 13
别名 KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13
计算分子量 50 kDa
GenBank蛋白编号BC002661
基因名称 Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_3670786
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13646
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

实验方案

Product Specific Protocols
IF protocol for Cytokeratin 13 antibody 83058-4-RRDownload protocol
FC protocol for Cytokeratin 13 antibody 83058-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols