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- KD/KO Validated
Cytokeratin 14 Recombinant antibody
Cytokeratin 14 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1L19
验证数据展示
经过测试的应用
Positive WB detected in | mouse skin tissue, A431 cells, rat skin tissue |
Positive IHC detected in | human oesophagus cancer tissue, mouse skin tissue, Human bowens disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82824-1-RR targets Cytokeratin 14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Cytokeratin 14 fusion protein Ag17559 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | keratin 14 |
Synonyms | KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14 |
Calculated Molecular Weight | 472 aa, 52 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC002690 |
Gene Symbol | Cytokeratin 14 |
Gene ID (NCBI) | 3861 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P02533 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 14 antibody 82824-1-RR | Download protocol |
IHC protocol for Cytokeratin 14 antibody 82824-1-RR | Download protocol |
IF protocol for Cytokeratin 14 antibody 82824-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |