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Cytokeratin 14 Recombinant antibody

Cytokeratin 14 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H22

Cat no : 83379-1-RR

Print datasheet

Synonyms

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

验证数据展示

  • WB analysis of rat skin using 83379-1-RR

    WB analysis of rat skin using 83379-1-RR

    rat skin tissue were subjected to SDS PAGE followed by western blot with 83379-1-RR (KRT14 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83379-1-RR

    Affinity and Kinetic Characterization of 83379-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83379-1-RR against Human Cytokeratin 14 were performed. The affinity constant is 43.1 nM.

经过测试的应用

Positive WB detected inrat skin tissue
Planning an IHC experiment? We recommend our IHCeasy KRT14 Ready-To-Use IHC Kit. KRT14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83379-1-RR is Cytokeratin 14 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 14 fusion protein Ag0188 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name keratin 14
Synonyms KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14
Calculated Molecular Weight 472 aa, 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC002690
Gene Symbol Cytokeratin 14
Gene ID (NCBI) 3861
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytokeratin 14, one of about 20 different cytokeratin isotypes of human cells, is the intermediate filament protein characteristic of epithelial cells. Cytokeratin 14 is expressed in the basal compartment of all stratified squamous epithelia. In various kinds of human tumors, the appearance and increasing expression of Cytokeratin 14 were strikingly associated with higher grade and stage of carcinoma, with varying degrees of unfavorable prognosis. In lung squamous cell carcinoma(LSCC), Cytokeratin 14 was expressed in the tumor cell nests showing stromal invasion with fibrosis and lymph node metastases, indicating that Cytokeratin 14 involved in proliferation and metastasis of LSCC. Cytokeratin 14 expression is sometimes used in diagnosis of myoepithelioma1 and intraductal vs. invasive ductal carcinoma of the breast.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 14 antibody 83379-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols