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  • Featured Product
  • KD/KO Validated

Cytokeratin 16 Monoclonal antibody, PBS Only

Cytokeratin 16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4D8

Cat no : 66802-1-PBS

Print datasheet

Synonyms

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK

验证数据展示

  • WB analysis of A431 using 66802-1-Ig (same clone as 66802-1-PBS)

    WB analysis of A431 using 66802-1-Ig (same clone as 66802-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

    IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT16 antibody included
  • IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

    IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT16 antibody included
  • IF Staining of A431 using 66802-1-Ig (same clone as 66802-1-PBS)

    IF Staining of A431 using 66802-1-Ig (same clone as 66802-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Cytokeratin 16 antibody (66802-1-Ig, Clone: 2H4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT16 Ready-To-Use IHC Kit. KRT16 primary antibody included.

产品信息

66802-1-PBS targets Cytokeratin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Cytokeratin 16 fusion protein Ag11240 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 16
Synonyms CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK
Calculated Molecular Weight 473 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC039169
Gene Symbol Cytokeratin 16
Gene ID (NCBI) 3868
RRIDAB_2882145
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08779
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.