Cytokeratin 6A Monoclonal antibody
Cytokeratin 6A Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2H6A11
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy KRT6 Ready-To-Use IHC Kit. KRT6 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 2 publications below |
产品信息
66685-1-Ig targets Cytokeratin 6A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | Cytokeratin 6A fusion protein Ag0882 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | keratin 6A |
| Synonyms | Cytokeratin 6, KRT6A, CK-6A, CK6A, CK-6 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC008807 |
| Gene Symbol | Cytokeratin 6A |
| Gene ID (NCBI) | 3853 |
| RRID | AB_2882039 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02538 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A, 6B and 6C. They share more than 99% identical DNA sequence.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 6A antibody 66685-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 6A antibody 66685-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Alteration of tumor-associated macrophage subtypes mediated by KRT6A in pancreatic ductal adenocarcinoma. | ||
Cell Tissue Res Sodium alginate/collagen hydrogel loaded with human umbilical cord mesenchymal stem cells promotes wound healing and skin remodeling. |