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验证数据展示

  • WB analysis using 68296-1-Ig (same clone as 68296-1-PBS)

    WB analysis using 68296-1-Ig (same clone as 68296-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IF Staining of mouse liver using 68296-1-Ig (same clone as 68296-1-PBS)

    IF Staining of mouse liver using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7 antibody (68296-1-Ig, Clone: 3G2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 68296-1-Ig (same clone as 68296-1-PBS)

    IF Staining of mouse liver using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7 antibody (68296-1-Ig, Clone: 3G2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

Cytokeratin 7 Monoclonal antibody, PBS Only

Cytokeratin 7 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68296-1-PBS

产品说明书

CloneNo. 3G2H1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7, CK7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.

产品信息

68296-1-PBS targets Cytokeratin 7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Cytokeratin 7 fusion protein Ag7895 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 7
别名 KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7, CK7
计算分子量 469 aa, 51 kDa
GenBank蛋白编号BC002700
基因名称 Cytokeratin 7
Gene ID (NCBI) 3855
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08729
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is a marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.