• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Cytokeratin 7 Monoclonal antibody, PBS Only

Cytokeratin 7 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3G2H1

Cat no : 68296-1-PBS

Print datasheet

Synonyms

KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7, CK7

验证数据展示

  • WB analysis using 68296-1-Ig (same clone as 68296-1-PBS)

    WB analysis using 68296-1-Ig (same clone as 68296-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IF Staining of mouse liver using 68296-1-Ig (same clone as 68296-1-PBS)

    IF Staining of mouse liver using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7 antibody (68296-1-Ig, Clone: 3G2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 68296-1-Ig (same clone as 68296-1-PBS)

    IF Staining of mouse liver using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7 antibody (68296-1-Ig, Clone: 3G2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.

产品信息

68296-1-PBS targets Cytokeratin 7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Cytokeratin 7 fusion protein Ag7895 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 7
Synonyms KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7, CK7
Calculated Molecular Weight 469 aa, 51 kDa
GenBank Accession NumberBC002700
Gene Symbol Cytokeratin 7
Gene ID (NCBI) 3855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08729
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is a marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.