验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy CK8 Ready-To-Use IHC Kit. CK8 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60274-1-Ig targets Cytokeratin 8 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | keratin 8 |
| 别名 | CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | NM_002273 |
| 基因名称 | Cytokeratin 8 |
| Gene ID (NCBI) | 3856 |
| RRID | AB_2881394 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05787 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 8 antibody 60274-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |