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D2HGDH Monoclonal antibody, PBS Only

D2HGDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9B3

Cat no : 66364-1-PBS

Print datasheet

Synonyms

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD, 1E9B3

验证数据展示

  • FC experiment of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    FC experiment of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human D2HGDH (66364-1-Ig, Clone:1E9B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66364-1-Ig (same clone as 66364-1-PBS)

    WB analysis of NIH/3T3 using 66364-1-Ig (same clone as 66364-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

产品信息

66364-1-PBS targets D2HGDH in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen D2HGDH fusion protein Ag5036 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name D-2-hydroxyglutarate dehydrogenase
Synonyms EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD, 1E9B3
Calculated Molecular Weight 521 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC036604
Gene Symbol D2HGDH
Gene ID (NCBI) 728294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N465
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).