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验证数据展示

  • FC experiment of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    FC experiment of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human D2HGDH (66364-1-Ig, Clone:1E9B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66364-1-Ig (same clone as 66364-1-PBS)

    WB analysis of NIH/3T3 using 66364-1-Ig (same clone as 66364-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

D2HGDH Monoclonal antibody, PBS Only

D2HGDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66364-1-PBS

产品说明书

CloneNo. 1E9B3

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD, 1E9B3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66364-1-PBS targets D2HGDH in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原 D2HGDH fusion protein Ag5036 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 D-2-hydroxyglutarate dehydrogenase
别名 EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD, 1E9B3
计算分子量 521 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC036604
基因名称 D2HGDH
Gene ID (NCBI) 728294
RRIDAB_2881744
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N465
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).