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DAAM1 Polyclonal antibody
DAAM1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
IF/ICC, IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, mouse heart tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14876-1-AP targets DAAM1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB,ELISA Application Description |
| Cited Applications | WB, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat, Xenopus, Canine |
| Immunogen | DAAM1 fusion protein Ag6648 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | dishevelled associated activator of morphogenesis 1 |
| Synonyms | DAAM1, KIAA0666 |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 123 kDa |
| GenBank Accession Number | BC064999 |
| Gene Symbol | DAAM1 |
| Gene ID (NCBI) | 23002 |
| RRID | AB_2089444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4D1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| IHC protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| IF protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| IP protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility.
| ||
Development Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis.
| ||
Cell Prolif Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer. | ||
Cell Signal miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer. | ||
Am J Physiol Cell Physiol Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4. |
