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DAAM1 Monoclonal antibody

DAAM1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3F11F11

Cat no : 67287-1-Ig

Print datasheet

Synonyms

KIAA0666, FLJ41657, Disheveled-associated activator of morphogenesis 1, 3F11F11

验证数据展示

  • WB analysis using 67287-1-Ig

    WB analysis using 67287-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 67287-1-Ig

    IHC staining of human skin cancer using 67287-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67287-1-Ig (DAAM1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, rat heart tissue, mouse heart tissue, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67287-1-Ig targets DAAM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DAAM1 fusion protein Ag6809 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dishevelled associated activator of morphogenesis 1
Synonyms KIAA0666, FLJ41657, Disheveled-associated activator of morphogenesis 1, 3F11F11
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC064999
Gene Symbol DAAM1
Gene ID (NCBI) 23002
RRIDAB_2882553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4D1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.

实验方案

Product Specific Protocols
WB protocol for DAAM1 antibody 67287-1-IgDownload protocol
IHC protocol for DAAM1 antibody 67287-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols