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验证数据展示

  • WB analysis using 31459-1-AP

    WB analysis using 31459-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31459-1-AP

    WB analysis using 31459-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 31459-1-AP

    IF Staining of HepG2 using 31459-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAB1 antibody (31459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DAB1 Polyclonal antibody

DAB1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31459-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IF/ICC, ELISA

别名

Disabled homolog 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig cerebellum tissue, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31459-1-AP targets DAB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原 DAB1 fusion protein Ag35376 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 disabled homolog 1 (Drosophila)
别名 Disabled homolog 1
计算分子量64 kDa
观测分子量45 kDa, 80 kDa
GenBank蛋白编号NM_021080
基因名称 DAB1
Gene ID (NCBI) 1600
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO75553
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Disabled-1 (Dab1) is an adaptor protein that is essential for neuronal migration and maturation in response to the extracellular protein Reelin. DAB1 protein docks to the intracellular part of the Reelin very low density lipoprotein receptor and apoE receptor type 2 and becomes tyrosine-phosphorylated following binding of Reelin to cortical neurons. The DAB1 protein contains a 180-amino acid N-terminal protein interaction/phosphotyrosine-binding (PTB) 1 domain that docks to the short cytoplasmic tail of the very low density lipoprotein receptor or apoE receptor type 2 at the level of NPXY motifs, with a preference for unphosphorylated motifs. Dab1 polypeptide bands ranging from 36 to 120 kDa have been identified in mouse embryonic brain, the 80-kDa Dab1 being the predominant form (PMID: 24313315).

实验方案

Product Specific Protocols
WB protocol for DAB1 antibody 31459-1-APDownload protocol
IF protocol for DAB1 antibody 31459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols