验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy DAB2IP Ready-To-Use IHC Kit. DAB2IP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23582-1-AP targets DAB2IP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | DAB2IP fusion protein Ag20308 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DAB2 interacting protein |
| 别名 | AF9Q34, AIP1, ASK interacting protein 1, DAB2 interacting protein, DAB2 interaction protein, DAB2IP, DIP1/2, KIAA1743 |
| 计算分子量 | 1189 aa, 132 kDa |
| 观测分子量 | 118 kDa |
| GenBank蛋白编号 | BC146762 |
| 基因名称 | DAB2IP |
| Gene ID (NCBI) | 153090 |
| RRID | AB_2879300 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q5VWQ8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DAB2IP antibody 23582-1-AP | Download protocol |
| IP protocol for DAB2IP antibody 23582-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Sci DOC-2/DAB2 interactive protein destabilizes c-Myc to impair the growth and self-renewal of colon tumor-repopulating cells. | ||
Biochim Biophys Acta Mol Basis Dis DNMT3A facilitates colorectal cancer progression via regulating DAB2IP mediated MEK/ERK activation. | ||
Cell Death Dis Soft fibrin matrix downregulates DAB2IP to promote Nanog-dependent growth of colon tumor-repopulating cells. |