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验证数据展示

  • WB analysis using 30020-1-AP

    WB analysis using 30020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30020-1-AP

    WB analysis using 30020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of 4T1 using 30020-1-AP

    IP experiment of 4T1 using 30020-1-AP

    IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 2800 ug.

  • IP experiment of 4T1 using 30020-1-AP

    IP experiment of 4T1 using 30020-1-AP

    IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 4800 ug.

DAB2IP Polyclonal antibody

DAB2IP Polyclonal Antibody for WB, IP, ELISA
Cat No. 30020-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

DAB2 interacting protein, ASK-interacting protein 1, ASK interacting protein 1, AIP-1, AIP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, rat brain tissue
Positive IP detected in4T1 cells
Planning an IHC experiment? We recommend our IHCeasy DAB2IP Ready-To-Use IHC Kit. DAB2IP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30020-1-AP targets DAB2IP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DAB2IP fusion protein Ag31340 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DAB2 interacting protein
别名 DAB2 interacting protein, ASK-interacting protein 1, ASK interacting protein 1, AIP-1, AIP1
计算分子量 1189 aa, 132 kDa
观测分子量 125-132 kDa
GenBank蛋白编号BC146762
基因名称 DAB2IP
Gene ID (NCBI) 153090
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VWQ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Disabled homolog 2-interacting protein (DAB2IP) is also named as ASK-interacting protein 1 (AIP-1). DAB2IP, is a new member of the Ras GTPase-activating protein family. It interacts directly with DAB2 and has distinct cellular functions such as modulating different signal cascades associated with cell proliferation, survival, apoptosis and metastasis. The aberrant alteration of DAB2IP in cancer is caused by a variety of mechanisms, including the aberrant promoter methylation, histone deacetylation, and others (PMID: 26658103). DAB2IP is reported to bind with Apoptosis signal-regulating kinase 1 (ASK1), and is called ASK1 Interacting Protein (AIP1) (PMID: 12813029). DAB2IP function is that of a tumor suppressor in tumor cell growth, metastasis, and other aspects in cancer progression. In prostate cancer, DAB2IP expression was repressed by promoter methylation and histone modification (PMID: 12446720, PMID: 15817459).

实验方案

Product Specific Protocols
WB protocol for DAB2IP antibody 30020-1-APDownload protocol
IP protocol for DAB2IP antibody 30020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols