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验证数据展示

  • WB analysis using 10531-1-AP

    WB analysis using 10531-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10531-1-AP (DAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 10531-1-AP

    IP experiment of HepG2 using 10531-1-AP

    IP result of anti-DAD1 (IP:10531-1-AP, 4ug; Detection:10531-1-AP 1:700) with HepG2 cells lysate 2600 ug.

  • IHC staining of human liver cancer using 10531-1-AP

    IHC staining of human liver cancer using 10531-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10531-1-AP (DAD1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 10531-1-AP

    IHC staining of human liver cancer using 10531-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10531-1-AP (DAD1 Antibody) at dilution of 1:200 (under 40x lens).

DAD1 Polyclonal antibody

DAD1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 10531-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, DAD-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10531-1-AP targets DAD1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 DAD1 fusion protein Ag0814 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 defender against cell death 1
别名 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, DAD-1
计算分子量 12 kDa
观测分子量 16-20 kDa
GenBank蛋白编号BC007403
基因名称 DAD1
Gene ID (NCBI) 1603
RRIDAB_2089878
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61803
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DAD1 (defender against cell death 1) is a downstream target of the NFkB survival pathway and exhibits an antiapoptotic function. It is also named as OST2 (Oligosaccharyl transferase2) and catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Increased expression of DAD1 has been observed in various tumors.

实验方案

Product Specific Protocols
WB protocol for DAD1 antibody 10531-1-APDownload protocol
IHC protocol for DAD1 antibody 10531-1-APDownload protocol
IP protocol for DAD1 antibody 10531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols