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Dystroglycan Polyclonal antibody
Dystroglycan Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse skeletal muscle tissue, mouse brain tissue, HeLa cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse heart tissue, mouse skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11017-1-AP targets Dystroglycan in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, CoIP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | Dystroglycan fusion protein Ag1456 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | dystroglycan 1 (dystrophin-associated glycoprotein 1) |
Synonyms | DAG1, DAG, Beta DG, Alpha DG Beta dystroglycan, AGRNR |
Calculated Molecular Weight | 97 kDa |
Observed Molecular Weight | 40-43 kDa |
GenBank Accession Number | BC012740 |
Gene Symbol | Dystroglycan |
Gene ID (NCBI) | 1605 |
RRID | AB_2090032 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14118 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
IHC protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
IF protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
IP protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
FC protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models. | ||
Transl Res AAV-based gene therapy prevents and halts the progression of dilated cardiomyopathy in a mouse model of phosphoglucomutase I deficiency (PGM1-CDG) | ||
Mol Oncol Circular RNA circ-TNRC6B inhibits the proliferation and invasion of esophageal squamous cell carcinoma cells by regulating the miR-452-5p/DAG1 axis | ||
Cell Commun Signal Wnt-driven LARGE2 mediates laminin-adhesive O-glycosylation in human colonic epithelial cells and colorectal cancer. | ||
Front Cardiovasc Med Determination of Agrin and Related Proteins Levels as a Function of Age in Human Hearts. | ||
J Med Virol Identification of potential plasma markers for hepatitis B virus-related chronic hepatitis and liver fibrosis/cirrhosis. |