验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse skeletal muscle tissue, mouse brain tissue, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse heart tissue, mouse skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11017-1-AP targets Dystroglycan in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Dystroglycan fusion protein Ag1456 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dystroglycan 1 (dystrophin-associated glycoprotein 1) |
| 别名 | DAG1, DAG, Beta DG, Alpha DG Beta dystroglycan, AGRNR |
| 计算分子量 | 97 kDa |
| 观测分子量 | 40-43 kDa |
| GenBank蛋白编号 | BC012740 |
| 基因名称 | Dystroglycan |
| Gene ID (NCBI) | 1605 |
| RRID | AB_2090032 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14118 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
| IHC protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
| IF protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
| IP protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
| FC protocol for Dystroglycan antibody 11017-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models. | ||
Transl Res AAV-based gene therapy prevents and halts the progression of dilated cardiomyopathy in a mouse model of phosphoglucomutase I deficiency (PGM1-CDG) | ||
Mol Oncol Circular RNA circ-TNRC6B inhibits the proliferation and invasion of esophageal squamous cell carcinoma cells by regulating the miR-452-5p/DAG1 axis | ||
Cell Commun Signal Wnt-driven LARGE2 mediates laminin-adhesive O-glycosylation in human colonic epithelial cells and colorectal cancer. | ||
Front Cardiovasc Med Determination of Agrin and Related Proteins Levels as a Function of Age in Human Hearts. | ||
J Med Virol Identification of potential plasma markers for hepatitis B virus-related chronic hepatitis and liver fibrosis/cirrhosis. |
