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DALRD3 Polyclonal antibody

DALRD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26294-1-AP

Print datasheet

Synonyms

DALRD3

验证数据展示

  • WB analysis of COLO 320 using 26294-1-AP

    WB analysis of COLO 320 using 26294-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26294-1-AP (DALRD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 26294-1-AP

    IHC staining of human colon cancer using 26294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26294-1-AP (DALRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 26294-1-AP

    IHC staining of human colon cancer using 26294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26294-1-AP (DALRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 26294-1-AP

    WB analysis of K-562 using 26294-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26294-1-AP (DALRD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26294-1-AP targets DALRD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen DALRD3 fusion protein Ag23437 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DALR anticodon binding domain containing 3
Synonyms DALRD3
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC047683
Gene Symbol DALRD3
Gene ID (NCBI) 55152
RRIDAB_2880467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5D0E6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DALRD3 antibody 26294-1-APDownload protocol
IHC protocol for DALRD3 antibody 26294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

DALRD3 encodes a protein mutated in epileptic encephalopathy that targets arginine tRNAs for 3-methylcytosine modification.

Authors - Jenna M Lentini
View Article