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验证数据展示

  • WB analysis of human heart using 22137-1-AP

    WB analysis of human heart using 22137-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 22137-1-AP (DAND5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22137-1-AP

    IHC staining of human testis using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

  • IHC staining of human testis using 22137-1-AP

    IHC staining of human testis using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

DAND5 Polyclonal antibody

DAND5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22137-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CER2, Cerberus like protein 2, Cerl 2, CERL2, CKTSF1B3, COCO, CRL2, DAN domain family member 5, DAN domain family, member 5, DAND5, DANTE, GREM3, Gremlin 3, SP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22137-1-AP targets DAND5 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 DAND5 fusion protein Ag17533 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DAN domain family, member 5
别名 CER2, Cerberus like protein 2, Cerl 2, CERL2, CKTSF1B3, COCO, CRL2, DAN domain family member 5, DAN domain family, member 5, DAND5, DANTE, GREM3, Gremlin 3, SP1
计算分子量 189 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC113476
基因名称 DAND5
Gene ID (NCBI) 199699
RRIDAB_2879004
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N907
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DAND5 antibody 22137-1-APDownload protocol
IHC protocol for DAND5 antibody 22137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols