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DAND5 Polyclonal antibody

DAND5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22137-1-AP

Print datasheet

Synonyms

CER2, Cerberus like protein 2, Cerl 2, CERL2, CKTSF1B3, COCO, CRL2, DAN domain family member 5, DAN domain family, member 5, DAND5, DANTE, GREM3, Gremlin 3, SP1

验证数据展示

  • WB analysis of human heart using 22137-1-AP

    WB analysis of human heart using 22137-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 22137-1-AP (DAND5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22137-1-AP

    IHC staining of human testis using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

  • IHC staining of human testis using 22137-1-AP

    IHC staining of human testis using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22137-1-AP is DAND5 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen DAND5 fusion protein Ag17533 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DAN domain family, member 5
Synonyms CER2, Cerberus like protein 2, Cerl 2, CERL2, CKTSF1B3, COCO, CRL2, DAN domain family member 5, DAN domain family, member 5, DAND5, DANTE, GREM3, Gremlin 3, SP1
Calculated Molecular Weight 189 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC113476
Gene Symbol DAND5
Gene ID (NCBI) 199699
RRIDAB_2879004
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DAND5 antibody 22137-1-APDownload protocol
IHC protocol for DAND5 antibody 22137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols