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DAO Monoclonal antibody

DAO Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C12F4

Cat no : 66296-1-Ig

Print datasheet

Synonyms

D amino acid oxidase, DAAO, DAMOX, DAO, OXDA

验证数据展示

  • WB analysis using 66296-1-Ig

    WB analysis using 66296-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66296-1-Ig (DAO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human gliomas using 66296-1-Ig

    IHC staining of human gliomas using 66296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66296-1-Ig (DAO Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IHC staining of human gliomas using 66296-1-Ig

    IHC staining of human gliomas using 66296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66296-1-Ig (DAO Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

经过测试的应用

Positive WB detected inpig brain tissue, fetal human brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inhuman gliomas tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66296-1-Ig targets DAO in WB, IHC, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, pig, rat
Immunogen DAO fusion protein Ag6928 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name D-amino-acid oxidase
Synonyms D amino acid oxidase, DAAO, DAMOX, DAO, OXDA
Calculated Molecular Weight 347 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC029057
Gene Symbol DAO
Gene ID (NCBI) 1610
RRIDAB_2881679
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14920
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging.DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639).

实验方案

Product Specific Protocols
WB protocol for DAO antibody 66296-1-IgDownload protocol
IHC protocol for DAO antibody 66296-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols