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DAP3 Polyclonal antibody

DAP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10276-1-AP

Print datasheet

Synonyms

MRPS29, MRP S29, Death-associated protein 3, DAP-3, DAP 3

验证数据展示

  • WB analysis of HeLa using 10276-1-AP

    WB analysis of HeLa using 10276-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 10276-1-AP

    IHC staining of human colon using 10276-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10276-1-AP (DAP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach using 10276-1-AP

    IHC staining of human stomach using 10276-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10276-1-AP (DAP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10276-1-AP targets DAP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen DAP3 fusion protein Ag0274 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name death associated protein 3
Synonyms MRPS29, MRP S29, Death-associated protein 3, DAP-3, DAP 3
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBF220193
Gene Symbol DAP3
Gene ID (NCBI) 7818
RRIDAB_10695891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51398
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DAP3 antibody 10276-1-APDownload protocol
IHC protocol for DAP3 antibody 10276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Noncoding RNA Res

DNA methylation of microRNA-365-1 induces apoptosis of hair follicle stem cells by targeting DAP3

Authors - Xin Liu
View Article