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验证数据展示

  • WB analysis of HepG2 using 19938-1-AP

    WB analysis of HepG2 using 19938-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19938-1-AP (DAPK1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 19938-1-AP

    IHC staining of human small intestine using 19938-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19938-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with DAPK1 antibody included
  • IHC staining of human small intestine using 19938-1-AP

    IHC staining of human small intestine using 19938-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19938-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with DAPK1 antibody included

DAPK1 Polyclonal antibody

DAPK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19938-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

DAP kinase 1, DAPK, DAPK1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DAPK1 Ready-To-Use IHC Kit. DAPK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19938-1-AP targets DAPK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityrat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name death-associated protein kinase 1
Synonyms DAP kinase 1, DAPK, DAPK1
Calculated Molecular Weight 160 kDa
GenBank Accession NumberNM_004938
Gene Symbol DAPK1
Gene ID (NCBI) 1612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53355
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DAPK1, also named DAPK, belongs to the protein kinase superfamily; CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK1 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes isoform1 of DAPK1.

实验方案

Product Specific Protocols
WB protocol for DAPK1 antibody 19938-1-APDownload protocol
IHC protocol for DAPK1 antibody 19938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

J Tradit Complement Med

Effect of electrode configuration in electroacupuncture on ischemic stroke treatment in rats

Authors - Chung-Hsiang Liu
View Article