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DAPK1 Monoclonal antibody, PBS Only

DAPK1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2F9

Cat no : 67815-1-PBS

Print datasheet

Synonyms

EC:2.7.11.1, Death-associated protein kinase 1, DAPK, DAP kinase 1, 1E2F9

验证数据展示

  • WB analysis using 67815-1-Ig (same clone as 67815-1-PBS)

    WB analysis using 67815-1-Ig (same clone as 67815-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67815-1-Ig (DAPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    IHC staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAPK1 antibody included
  • IF Staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    IF Staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-1376 using 67815-1-Ig (same clone as 67815-1-PBS)

    IF Staining of HT-1376 using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-1376 cells using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    IF Staining of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • FC experiment of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    FC experiment of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    1X10^6 HCT 116 cells were intracellularly stained with 0.4 ug Anti-Human DAPK1 (67815-1-Ig, Clone:1E2F9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DAPK1 Ready-To-Use IHC Kit. DAPK1 primary antibody included.

产品信息

67815-1-PBS targets DAPK1 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DAPK1 fusion protein Ag29838 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name death-associated protein kinase 1
Synonyms EC:2.7.11.1, Death-associated protein kinase 1, DAPK, DAP kinase 1, 1E2F9
Calculated Molecular Weight 1430 aa, 160 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC113660
Gene Symbol DAPK1
Gene ID (NCBI) 1612
RRIDAB_2918578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP53355
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784).