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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67815-1-Ig (same clone as 67815-1-PBS)

    WB analysis using 67815-1-Ig (same clone as 67815-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67815-1-Ig (DAPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    IHC staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAPK1 antibody included
  • IF Staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    IF Staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-1376 using 67815-1-Ig (same clone as 67815-1-PBS)

    IF Staining of HT-1376 using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-1376 cells using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    IF Staining of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • FC experiment of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    FC experiment of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    1X10^6 HCT 116 cells were intracellularly stained with 0.4 ug Anti-Human DAPK1 (67815-1-Ig, Clone:1E2F9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

DAPK1 Monoclonal antibody, PBS Only

DAPK1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 67815-1-PBS

产品说明书

CloneNo. 1E2F9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

EC:2.7.11.1, Death-associated protein kinase 1, DAPK, DAP kinase 1, 1E2F9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DAPK1 Ready-To-Use IHC Kit. DAPK1 primary antibody included.

产品信息

67815-1-PBS targets DAPK1 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DAPK1 fusion protein Ag29838 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 death-associated protein kinase 1
别名 EC:2.7.11.1, Death-associated protein kinase 1, DAPK, DAP kinase 1, 1E2F9
计算分子量 1430 aa, 160 kDa
观测分子量160 kDa
GenBank蛋白编号BC113660
基因名称 DAPK1
Gene ID (NCBI) 1612
RRIDAB_2918578
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP53355
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784).