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验证数据展示

  • WB analysis using 30656-1-AP

    WB analysis using 30656-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30656-1-AP (DAPK3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30656-1-AP

    IF Staining of A431 using 30656-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAPK3 antibody (30656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

DAPK3 Polyclonal antibody

DAPK3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30656-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Synonyms

ZIP kinase, EC:2.7.11.1, Dlk, Death-associated protein kinase 3, DAP-like kinase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, Hela cells, HepG2 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy DAPK3 Ready-To-Use IHC Kit. DAPK3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30656-1-AP targets DAPK3 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human
Cited Reactivitymouse
Immunogen DAPK3 fusion protein Ag33051 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name death-associated protein kinase 3
Synonyms ZIP kinase, EC:2.7.11.1, Dlk, Death-associated protein kinase 3, DAP-like kinase
Calculated Molecular Weight 53 kDa
Observed Molecular Weight37, 52 kDa
GenBank Accession NumberNM_001348
Gene Symbol DAPK3
Gene ID (NCBI) 1613
RRIDAB_3086381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43293
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DAPK3, also named as ZIPK and Dlk, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. The calculated molecular weight of DAPK3 is 52 kDa. This antibody can recognize the 37 kDa isoform of target. ZIP kinase is a Serine/threonine kinase which acts as a positive regulator of apoptosis. ZIP kinasephosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. ZIP kinase regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.

实验方案

Product Specific Protocols
WB protocol for DAPK3 antibody 30656-1-APDownload protocol
IF protocol for DAPK3 antibody 30656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,CoIP

Biochem Biophys Res Commun

Protection of blood-brain barrier by endothelial DAPK1 deletion after stroke

Authors - Zhijiang Gu
View Article