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DARS Polyclonal antibody

DARS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14989-1-AP

Print datasheet

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS

验证数据展示

  • WB analysis using 14989-1-AP

    WB analysis using 14989-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14989-1-AP (DARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14989-1-AP

    IP experiment of HeLa using 14989-1-AP

    IP result of anti-DARS (IP:14989-1-AP, 4ug; Detection:14989-1-AP 1:5000) with HeLa cells lysate 2160 ug.

  • IHC staining of rat brain using 14989-1-AP

    IHC staining of rat brain using 14989-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14989-1-AP (DARS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DARS1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DARS1 Ready-To-Use IHC Kit. DARS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14989-1-AP targets DARS in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DARS fusion protein Ag6974 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aspartyl-tRNA synthetase
Synonyms Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberBC000629
Gene Symbol DARS
Gene ID (NCBI) 1615
RRIDAB_2261349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14868
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DARS antibody 14989-1-APDownload protocol
IHC protocol for DARS antibody 14989-1-APDownload protocol
IP protocol for DARS antibody 14989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseCoIP

J Virol

Multiple Host Factors Interact with Hypervariable Domain of Chikungunya Virus nsP3 and Determine Viral Replication in Cell-Specific Mode.

Authors - Chetan D Meshram
View Article
humanWB

Am J Cancer Res

hsa_circ_0000519 promotes the progression of lung adenocarcinoma through the hsa-miR-1296-5p/DARS axis

Authors - Guangxu Tu
View Article