验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, HeLa cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DAXX Ready-To-Use IHC Kit. DAXX primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
20489-1-AP targets DAXX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | DAXX fusion protein Ag14519 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | death-domain associated protein |
| 别名 | ETS1 associated protein 1, EAP1, Death domain-associated protein 6, Death domain associated protein 6, DAP6 |
| 计算分子量 | 740 aa, 81 kDa |
| 观测分子量 | 120 kDa, 70 kDa |
| GenBank蛋白编号 | BC109074 |
| 基因名称 | DAXX |
| Gene ID (NCBI) | 1616 |
| RRID | AB_10693620 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UER7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DAXX antibody 20489-1-AP | Download protocol |
| IHC protocol for DAXX antibody 20489-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen. | ||
J Biol Chem The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation. | ||
