验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, human testis tissue, rat testis tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14065-1-AP targets DAZ4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | DAZ4 fusion protein Ag5116 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | deleted in azoospermia 4 |
| 别名 | pDP1681, pDP1680, DAZ1, DAZ 4, DAZ |
| 计算分子量 | 65 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC047617 |
| 基因名称 | DAZ4 |
| Gene ID (NCBI) | 57135 |
| RRID | AB_2878008 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86SG3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DAZ4, encoded by the DAZ gene, is an RNA-binding protein with a role in spermatogenesis. DAZ4 is a testis-specific protein that expression restricted to premeiotic germ cells, particularly in spermatogonia. Defects in DAZ4 may be a cause of spermatogenic failure Y-linked type 2 (SPGFY2). It is a disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DAZ4 antibody 14065-1-AP | Download protocol |
| IHC protocol for DAZ4 antibody 14065-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
