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DBF4B Polyclonal antibody

DBF4B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26214-1-AP

Print datasheet

Synonyms

ASK like protein 1, ASKL1, chifb, Chiffon homolog B, DBF4 homolog B (S. cerevisiae), Dbf4 related factor 1, DBF4B, DRF1, Protein DBF4 homolog B, ZDBF1B

验证数据展示

  • WB analysis using 26214-1-AP

    WB analysis using 26214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26214-1-AP (DBF4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells, T-47D cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26214-1-AP targets DBF4B in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen DBF4B fusion protein Ag23889 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DBF4 homolog B (S. cerevisiae)
Synonyms ASK like protein 1, ASKL1, chifb, Chiffon homolog B, DBF4 homolog B (S. cerevisiae), Dbf4 related factor 1, DBF4B, DRF1, Protein DBF4 homolog B, ZDBF1B
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC016158
Gene Symbol DBF4B
Gene ID (NCBI) 80174
RRIDAB_3669528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cell-cycle progression is driven by orderly activation of many kinases including the DBF4/DBF4B-dependent S-phase promoting kinase Cdc7. Binding of Cdc7 to its subunit DBF4 or DBF4B (also known as DRF1/ASKL1) is thought to be a key regulatory event that controls Cdc7 activity in human cells.

实验方案

Product Specific Protocols
WB protocol for DBF4B antibody 26214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols