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DBNDD2 Polyclonal antibody

DBNDD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27623-1-AP

Print datasheet

Synonyms

C20orf35, CK1BP, DBNDD2, HSMNP1

验证数据展示

  • WB analysis of mouse brain using 27623-1-AP

    WB analysis of mouse brain using 27623-1-AP

    mouse and rat brain tissues were subjected to SDS PAGE followed by western blot with 27623-1-AP (DBNDD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 27623-1-AP

    WB analysis of SH-SY5Y using 27623-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27623-1-AP (DBNDD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 27623-1-AP

    IHC staining of rat brain using 27623-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27623-1-AP (DBNDD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 27623-1-AP

    IHC staining of rat brain using 27623-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27623-1-AP (DBNDD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27623-1-AP targets DBNDD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen DBNDD2 fusion protein Ag26356 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dysbindin (dystrobrevin binding protein 1) domain containing 2
Synonyms C20orf35, CK1BP, DBNDD2, HSMNP1
Calculated Molecular Weight 261 aa, 28 kDa
Observed Molecular Weight 28-29 kDa
GenBank Accession NumberBC001105
Gene Symbol DBNDD2
Gene ID (NCBI) 55861
RRIDAB_2880927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQY9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DBNDD2 antibody 27623-1-APDownload protocol
IHC protocol for DBNDD2 antibody 27623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cells

CK1BP Reduces α-Synuclein Oligomerization and Aggregation Independent of Serine 129 Phosphorylation.

Authors - Lea Elsholz
View Article